tnalysis Chinese Academy Of Agricultural sciences (IPCAAS). Xingcheng,

tnalysis Of small molecular weight secretory proteins from Collerorr/chnm
floeosporioides With the aims at discussing how secrctomics can bc screened and
malyzed through molecular and bioinformatical approaches In gaining knossledge about
mechanisms underpinning plant-microbe interactions. In Vitro experiments were
‘Onductcd in the Rcscnrch Institute Of Pomoltv, Chinese Academy Of Agricultural
sciences (IPCAAS). Xingcheng, Liaoning Province. People’s Republic of China from
March to September 2017. Thc results so far achieved opencd further window for deep
Lnalysis With reference 10 functional analysis Of secrelomics from different virulent
;trains of Co/le’olrychum, a one of the serious pathogen of different crops including apple
‘or sustainable production. The putati’,. secreted proteins in the current were identifig!l
hrough bioinformatics 1001s viz: SignalP 4 1 Server. TMHMM2_O and Procomp 9.0 10
yredict signal peptide cleavage, transmembrane helices and cellular locali’ation,
espectlvely. proteins that contain signal peptide cleavage sites, but not transmembrane
lices were selected as putative secreted proteins. Moreover. proteins of less than 250
unino acids in length were selecLcd. The putative alignment of small molecular weight
secretory proteins in the present study revealed initially about 2230 (100%) genes
;equences. The best predicted soluble secreted proteins revealed 288 (12.91%) and
ransmembrane helices 210 (9.42%) putative proteins when screened by Signalp 4 1 and
rMHMM2 0, rcspcctivcly. About 1 76 (7.0%) scqucnccs wcrc proteins targctcd at
:xtracellular compartment, obtained through cellular localized with ProtComp 9.0,
Zinallv. 31 different mutants were observed for further experimentations. The BLAST
search for the resulting motifs in NCBI and databascs was carried out,
phylogenetic relationships between different family members of “C proteins, various
Ilignment analysis was implemented AC domains ammo acid sequences. The alignment
las predicted that A3C. “C, A12C ,4C/5. ,426C A-RIC are compleLcly different from
.nch Other and have no any conscrvcd scqucnccg_ It was further confirmed by aligning
vith other species and C. g!oernsporioiåes associated members and revealed that no any