Enzymes are proteins that function as catalase within living cells. Catalase (catalysts) increase the rate in which a chemical reaction occurs without undergoing the reaction itself. During the catalyzed reaction, the substrate binds itself with the active site and as a result forms an enzyme-substrate complex with the enzyme. Subsequently, the enzyme will break the bonds in the substrate. The product then leaves the enzyme, which remains in its original form after the reaction. Catalase is an enzyme that produces catalysts in which Hydrogen peroxide is broken down to oxygen and to water. It is an enzyme that is produced in the liver in order to eliminate hydrogen peroxide. Hydrogen peroxide is toxic once it is accumulated in the body, but is a typical end product of metabolism.In this Lab, each group was provided with 3% of H2O2, and 15g of Saccharomyces Cerevisiae containing catalase enzyme on a filtered paper. The filtered papers are dipped into various concentrations of solution, in assumptions of it being submerged into the 2H2O2 solution. The reason for why the filtered paper started to rise, is due to the fact that the oxygen from the enzyme reaction had been formed on the filtered paper, and as a result made is rise faster. By going through this method, we can investigate the effects of the substrate reaction by using different masses of concentration on the rate of the reaction.The Hypothesis for this Lab is that the larger the concentration of Saccharomyces Cervisiae containing catalase enzymes on the filtered paper, the faster the concentration will rise, causing the filtered paper to float up faster. Therefore the rate of the reaction will increase, the more hydrogen peroxide is obtained on the filtered paper. However, after a specific amount of time, one investigates that the rate of the reaction will no longer increase, due to the fact that all the particles are engaged by the active site and therefore the rate of the reaction cannot be further accelerated. (enzyme concentration becomes a limiting factor).